5T4W
Crystal structure of a DNA sequence d (CGTGAATTCACG) with DAPI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2016-04-20 |
Detector | AGILENT ATLAS CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 24.503, 41.090, 65.184 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.760 - 2.300 |
R-factor | 0.2203 |
Rwork | 0.216 |
R-free | 0.25839 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ju4 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.559 |
Data reduction software | CrysalisPro |
Data scaling software | CrysalisPro |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 34.760 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.080 |
Number of reflections | 2915 |
<I/σ(I)> | 2.88 |
Completeness [%] | 90.0 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 60mM NaCaCo, 17mM MgCl2, 2mM Spermine, 50%MPD, 1.5 mM DAPI |