5T4M

Crystal Structure of Human Protocadherin-15 EC3-5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-08-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.98
Spacegroup name	P 65
Unit cell lengths	105.608, 105.608, 193.672
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.360 - 2.240
Rwork	0.189
R-free	0.22140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2o72
RMSD bond length	0.011
RMSD bond angle	0.833
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (dev_1760)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.360	2.320
High resolution limit [Å]	2.240	2.240
Rmerge	0.048	1.407
Number of reflections	57737
<I/σ(I)>	8.71	0.69
Completeness [%]	98.0	93
Redundancy	2.9	2.4
CC(1/2)	0.997	0.197

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	0.1 M Sodium Cacodylate pH 6.0, 150 mM Calcium Chloride