5T4J
PLP and GABA Trigger GabR-Mediated Transcription Regulation in Bacillus subsidies via External Aldimine Formation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-BM-B |
Synchrotron site | APS |
Beamline | 23-BM-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-08-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0332 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 92.364, 128.894, 65.096 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.183 - 2.231 |
R-factor | 0.1744 |
Rwork | 0.172 |
R-free | 0.21070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mgr |
RMSD bond length | 0.005 |
RMSD bond angle | 0.862 |
Data reduction software | HKL-2000 |
Refinement software | PHENIX (dev_1563) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.200 |
High resolution limit [Å] | 2.230 |
Number of reflections | 104767 |
<I/σ(I)> | 29.3 |
Completeness [%] | 99.8 |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1M Tris (pH:8.5) and 20% ethanol |