5T4G
Crystal structure of BhGH81 in complex with laminarin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-09 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.400, 97.190, 156.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 82.550 - 1.800 |
| R-factor | 0.1625 |
| Rwork | 0.162 |
| R-free | 0.17750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5t49 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.204 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.550 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.098 | 0.441 |
| Number of reflections | 85487 | |
| <I/σ(I)> | 12.7 | 4.4 |
| Completeness [%] | 92.2 | 88.3 |
| Redundancy | 6.4 | 6.1 |
| CC(1/2) | 0.996 | 0.887 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 291 | 1.4 M NaH2PO4, 0.2 M K2HPO4, 0.1 M citric acid |
