5T4E
Human DPP4 in complex with ligand 19a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-12-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 118.288, 125.897, 137.216 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 92.770 - 1.770 |
| R-factor | 0.1556 |
| Rwork | 0.154 |
| R-free | 0.18160 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1x70 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.15) |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.9.4) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 137.216 | 137.216 | 1.810 |
| High resolution limit [Å] | 1.770 | 7.890 | 1.770 |
| Rmerge | 0.023 | 0.504 | |
| Number of reflections | 200915 | ||
| <I/σ(I)> | 11.176 | 24.2 | 1.5 |
| Completeness [%] | 99.9 | 99.6 | 100 |
| Redundancy | 6.7 | 6 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.27-0.30 M sodium acetate 17-18% polyethylene glycol 3350 0.1 M tris hydrochloride pH 8.0 |
