5T4B
Human DPP4 in complex with a ligand 34a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-08-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 117.610, 125.680, 137.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.050 - 1.760 |
| R-factor | 0.1597 |
| Rwork | 0.158 |
| R-free | 0.18580 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1x70 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.9.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 55.000 | 54.048 | 1.850 |
| High resolution limit [Å] | 1.760 | 5.560 | 1.760 |
| Rmerge | 0.029 | 0.488 | |
| Number of reflections | 200317 | ||
| <I/σ(I)> | 18.4 | 19.7 | 1.6 |
| Completeness [%] | 99.4 | 99.8 | 98.8 |
| Redundancy | 6.7 | 6.4 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.27-0.30 M sodium acetate 17-18% polyethylene glycol 3350 0.1 M tris hydrochloride pH 8.0 |
