5T47
Crystal structure of the D. melanogaster eIF4E-eIF4G complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99992 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.295, 49.714, 58.627 |
| Unit cell angles | 102.70, 103.94, 116.08 |
Refinement procedure
| Resolution | 41.415 - 2.200 |
| R-factor | 0.2092 |
| Rwork | 0.208 |
| R-free | 0.23380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ue8 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.468 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2152)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.420 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.609 | |
| Number of reflections | 20740 | |
| <I/σ(I)> | 6.2 | 1.7 |
| Completeness [%] | 96.6 | 91 |
| Redundancy | 3 | 2.5 |
| CC(1/2) | 0.992 | 0.683 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.25 | 291 | 0.1 M TrisHCl pH 8.25, 0.2 M MgCl2, 32% PEG4000 |
