5T3X

3.9 Angstrom Crystal Structure of a Fully and Natively Glycosylated BG505 SOSIP.664 HIV-1 Env Trimer in Complex with the Broadly Neutralizing Antibodies IOMA and 10-1074.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	H 3
Unit cell lengths	217.509, 217.509, 156.009
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	80.630 - 3.900
R-factor	0.3004
Rwork	0.296
R-free	0.33110
Starting model (for MR)	4tvp 4fq2 4tnn 4xvs
RMSD bond length	0.003
RMSD bond angle	1.108
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER (1.10.1-2155)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.630	4.170
High resolution limit [Å]	3.900	3.900
Rmerge	0.350	2.850
Rpim	0.080	0.680
Number of reflections	25079
<I/σ(I)>	7.6	1.5
Completeness [%]	100.0	100
Redundancy	18.2	18.3
CC(1/2)	0.990	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM ammonium citrate tribasic pH 7.0 100 mM imidazole pH 7.0 20% PEG MME 2,000