5T3U
Crystal Structure of the PTS IIA protein associated with the fucose utilization operon from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-01-20 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 100.202, 100.202, 192.986 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.940 - 2.150 |
| R-factor | 0.20615 |
| Rwork | 0.204 |
| R-free | 0.25277 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.391 |
| Data reduction software | CrystalClear |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.150 |
| Rmerge | 0.060 |
| Number of reflections | 197784 |
| <I/σ(I)> | 15.9 |
| Completeness [%] | 95.2 |
| Redundancy | 7.67 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 15% PEG3350, 0.2M ammonium sulfate, 0.1M citrate pH 5.8 |
