5T3O
Crystal structure of the Phosphorybosylpyrophosphate synthetase II from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-27 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 115.970, 115.970, 207.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.390 - 2.200 |
| R-factor | 0.22875 |
| Rwork | 0.227 |
| R-free | 0.25443 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dkr |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.453 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.400 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.091 | |
| Number of reflections | 72677 | |
| <I/σ(I)> | 4.1075 | 2.01 |
| Completeness [%] | 100.0 | 99.96 |
| Redundancy | 11.43 | 6.55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 293 | 0.8M SA |
