5T3K
Fluorescence detection of RNA-ligand binding and crystal structure determination of ribosomal decoding site RNA using a heavy atom containing fluorescent ribonucleoside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-02-12 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.553, 86.956, 32.564 |
| Unit cell angles | 90.00, 94.14, 90.00 |
Refinement procedure
| Resolution | 26.020 - 2.142 |
| R-factor | 0.2028 |
| Rwork | 0.197 |
| R-free | 0.24650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.366 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (dev_2313) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.020 | 2.220 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Number of reflections | 7483 | |
| <I/σ(I)> | 11.32 | 4.25 |
| Completeness [%] | 77.6 | 46.7 |
| Redundancy | 2.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | 0.2mM RNA, 2.40-2.55M ammonium sulfate, 10mM magnesium acetate, 40mM2-(N-morpholino)ethanesulfonic acid (MES) buffer, pH 5.6 |
