5T38

Crystal Structure of the N-terminal domain of EvdMO1 with SAH bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 1 21 1
Unit cell lengths	51.427, 41.272, 56.465
Unit cell angles	90.00, 99.80, 90.00

Refinement procedure

Resolution	32.002 - 1.150
R-factor	0.1536
Rwork	0.153
R-free	0.16820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mgg
RMSD bond length	0.006
RMSD bond angle	0.984
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.170
High resolution limit [Å]	1.150	3.120	1.150
Rmerge	0.052	0.029	0.347
Number of reflections	79855
<I/σ(I)>	9.5
Completeness [%]	96.3	95.7	70
Redundancy	3.5	3.5	2.3
CC(1/2)		0.998	0.836

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	40% Tacsimate, pH 7.4