5T30
Human MnSOD-azide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-02-02 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 77.900, 77.900, 238.442 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 67.460 - 1.770 |
| R-factor | 0.21 |
| Rwork | 0.209 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.333 |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.500 | 1.800 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Number of reflections | 40981 | |
| <I/σ(I)> | 12.7 | 2 |
| Completeness [%] | 96.0 | 84 |
| Redundancy | 5.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 296 | 1.8M potassium phosphate, pH 7.8 |
