5T23
PI3Kg IN COMPLEX WITH 5d
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.92 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.150, 68.210, 106.810 |
| Unit cell angles | 90.00, 95.24, 90.00 |
Refinement procedure
| Resolution | 41.060 - 2.780 |
| R-factor | 0.25902 |
| Rwork | 0.255 |
| R-free | 0.33923 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.066 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER-TNT (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.770 | 2.850 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Rmerge | 0.500 | |
| Number of reflections | 26068 | |
| <I/σ(I)> | 19.1 | 2.1 |
| Completeness [%] | 98.8 | 99.1 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100mM Hepes pH 7.7-8.2, 18-19% PEG3350, 175mM (NH4)2SO4, 10mM DTT |
