5T1F
Crystal structure of Phaeospaeria nodrum fructosyl peptide oxidase mutant Asn56Ala
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-02 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.579, 93.327, 47.756 |
| Unit cell angles | 90.00, 98.41, 90.00 |
Refinement procedure
| Resolution | 39.140 - 1.980 |
| R-factor | 0.15987 |
| Rwork | 0.158 |
| R-free | 0.19535 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5t1e |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.847 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.086 | 0.496 |
| Number of reflections | 24157 | |
| <I/σ(I)> | 7.8 | 2 |
| Completeness [%] | 91.9 | 84.7 |
| Redundancy | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 8%(w/v) Tacsimate, 18-22%(w/v) PEG 3350 |
