5T1D

Crystal structure of EBV gHgL/gp42/E1D1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-29
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	I 2 2 2
Unit cell lengths	105.510, 166.000, 272.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.110 - 3.100
R-factor	0.2143
Rwork	0.212
R-free	0.26500
RMSD bond length	0.004
RMSD bond angle	0.634
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.110	3.005
High resolution limit [Å]	2.901	2.901
Rmerge	0.123	1.590
Number of reflections	53195
<I/σ(I)>	12.21	1.6
Completeness [%]	99.8	99.05
Redundancy	5.9	5.9
CC(1/2)	0.997	0.586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	0.1 M Tris pH 8.0, 0.18 M Potassium Citrate, 12 % PEG 6000