5T16
Crystal structure of yeast RNase III (Rnt1p) complexed with a non-hydrolyzable RNA substrate analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-06 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.037, 164.065, 176.855 |
| Unit cell angles | 90.00, 96.78, 90.00 |
Refinement procedure
| Resolution | 38.985 - 2.783 |
| R-factor | 0.2263 |
| Rwork | 0.226 |
| R-free | 0.26530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4oog |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2376: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.990 | 2.900 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Number of reflections | 86174 | |
| <I/σ(I)> | 6.79 | |
| Completeness [%] | 99.3 | 96 |
| Redundancy | 4.5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 3350, 0.2 M di ammonium citrate |
