5T0Y

2A10 Antibody FAB fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.032900
Spacegroup name	P 43 21 2
Unit cell lengths	231.684, 231.684, 81.784
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.733 - 3.011
R-factor	0.2259
Rwork	0.225
R-free	0.24670
RMSD bond length	0.002
RMSD bond angle	0.553
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.733	3.120
High resolution limit [Å]	3.010	3.010
Number of reflections	44655
<I/σ(I)>	10.6	1.8
Completeness [%]	99.9	99.5
Redundancy	14.6	14.6
CC(1/2)		0.703

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277.15	2M Ammonium Sulfate, 0.1 M Bis-Tris, pH 6.5