5T0O

Crystal Structure of a membrane protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	300.710, 147.538, 120.027
Unit cell angles	90.00, 99.87, 90.00

Refinement procedure

Resolution	84.930 - 3.150
R-factor	0.2139
Rwork	0.211
R-free	0.26780
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	3.260
High resolution limit [Å]	3.150	6.790	3.150
Rmerge	0.103	0.024	0.483
Total number of observations	251867
Number of reflections	158913
<I/σ(I)>	6.7
Completeness [%]	90.7	89.5	90.7
Redundancy	1.6	1.6	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	4% PEG8000, 0.1M MgSo4, 0.1M MES (6.5)