5T08

Crystal structure of H6 hemagglutinin G225D mutant from Taiwan (2013) H6N1 influenza virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97945
Spacegroup name	C 1 2 1
Unit cell lengths	185.065, 98.364, 133.239
Unit cell angles	90.00, 126.48, 90.00

Refinement procedure

Resolution	49.339 - 2.189
R-factor	0.2172
Rwork	0.215
R-free	0.25010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xkd
RMSD bond length	0.012
RMSD bond angle	1.635
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.189	2.189
Number of reflections	96720
<I/σ(I)>	19.1
Completeness [%]	98.5
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293.15	10mM NiCl2, 0.1M Tris pH 8.5, 20% (w/v) MPEG 2000 and 20% glycerol