5SZV
Novel Structural Insights into GDP-Mediated Regulation of Acyl-CoA Thioesterases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 152.240, 152.240, 152.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.880 - 2.000 |
| R-factor | 0.1714 |
| Rwork | 0.170 |
| R-free | 0.19680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ien |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.490 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.880 | 2.071 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.027 | 0.244 |
| Number of reflections | 79249 | |
| <I/σ(I)> | 17.22 | 3.01 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 24.2 | 24.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 296 | 100 mM Tris pH 8.5 and 2 M ammonium phosphate |
