5SZR

Protocadherin Gamma B2 extracellular cadherin domains 3-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97919
Spacegroup name	P 41 21 2
Unit cell lengths	104.755, 104.755, 352.136
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.949 - 2.300
R-factor	0.2513
Rwork	0.250
R-free	0.27790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dzv
RMSD bond length	0.004
RMSD bond angle	0.650
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.119	1.886
Number of reflections	87920
<I/σ(I)>	6	0.8
Completeness [%]	99.8	99.9
Redundancy	5.5	5.6
CC(1/2)	0.998	0.882

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		295	11.5% (w/v) PEG8000, 23% (v/v) ethylene glycol, 30 mM magnesium chloride, 30 mM calcium chloride, 0.1 M Morpheus Buffer System 1 (Mes/Imidazole buffer; Molecular Dimensions) pH 6.5