5SZO

Protocadherin Gamma B7 extracellular cadherin domains 1-4 P41212 crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-25
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97930
Spacegroup name	P 41 21 2
Unit cell lengths	97.150, 97.150, 312.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.930 - 3.612
R-factor	0.244
Rwork	0.242
R-free	0.27870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zpl 4zpm 4zpo 4zps 5dzv 5dzx 4zi9 4zi8 5zsm and 5SZN
RMSD bond length	0.003
RMSD bond angle	0.588
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	104.130	3.930
High resolution limit [Å]	3.590	3.590
Rmerge	0.183	3.722
Number of reflections	18347
<I/σ(I)>	9	1
Completeness [%]	99.6	98.7
Redundancy	12.5	12.3
CC(1/2)	1.000	0.741

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	8.5	295	0.1 M Tris-Cl pH 8.5, 0.2 M trimethylamine N-oxide, 3% dextran sulfate sodium salt 5000, 17% (w/v) PEG2000MME