5SZF

2A10 FAB fragment 2.54 Angstoms

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00000
Spacegroup name	I 41 3 2
Unit cell lengths	204.213, 204.213, 204.213
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.284 - 2.520
R-factor	0.2263
Rwork	0.225
R-free	0.24830
RMSD bond length	0.003
RMSD bond angle	0.568
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.200	2.520
High resolution limit [Å]	2.520
Rmerge	0.025	0.366
Number of reflections	24796
<I/σ(I)>	24.1
Completeness [%]	100.0
Redundancy	2	2.61
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	2M Ammonium sulfate 0.1 M trisodium citrate pH 5.5