5SYB
Crystal structure of human PHF5A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2016-06-12 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 82.116, 82.116, 82.116 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.060 - 1.820 |
| R-factor | 0.16979 |
| Rwork | 0.168 |
| R-free | 0.20289 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.981 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.100 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 318806 | |
| <I/σ(I)> | 15.2 | 2.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100mM CHES pH9.5 0.8mM Sodium Citrate 0.5% beta-OG |
