5SY8

Crystal structure of the complex of 10E8 Fab light chain mutant1 and T117v2 HIV-1 MPER scaffold

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	110
Detector technology	PIXEL
Collection date	2015-06-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	P 1
Unit cell lengths	41.404, 56.596, 64.196
Unit cell angles	101.75, 101.19, 92.17

Refinement procedure

Resolution	40.490 - 1.620
R-factor	0.174
Rwork	0.172
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g6f 3lf6
RMSD bond length	0.006
RMSD bond angle	1.032
Data reduction software	HKL-2000 (2.3.8)
Data scaling software	HKL-2000 (2.3.8)
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.490	1.650
High resolution limit [Å]	1.619	1.619
Rmerge		0.711
Number of reflections	61518
<I/σ(I)>	9.01	2.4
Completeness [%]	86.9	82
Redundancy	3.5	2.8
CC(1/2)	0.943	0.753

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	295.15	0.1 M Hepes pH 7.5, 20% PEG 8000