5SWM
BACILLUS HALODURANS RNASE H MUTANT D132N IN COMPLEX WITH 12-MER FRNA/DNA HYBRID
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-19 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.91836 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.474, 44.500, 62.174 |
| Unit cell angles | 84.42, 89.90, 65.10 |
Refinement procedure
| Resolution | 26.600 - 1.500 |
| R-factor | 0.162 |
| Rwork | 0.161 |
| R-free | 0.18500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID: 1ZBI |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.058 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.600 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.067 | 0.130 |
| Number of reflections | 55661 | |
| <I/σ(I)> | 46 | 10.6 |
| Completeness [%] | 95.7 | 79.8 |
| Redundancy | 6.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.4 M ammonium phosphate monobasic |
