5SWI

Crystal structure of SpGH92 in complex with mannose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97531
Spacegroup name	P 21 21 21
Unit cell lengths	130.003, 161.711, 208.404
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	127.760 - 2.150
R-factor	0.198
Rwork	0.197
R-free	0.22120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wvy
RMSD bond length	0.009
RMSD bond angle	1.225
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	127.760
High resolution limit [Å]	2.150
Rmerge	0.103
Number of reflections	237386
<I/σ(I)>	14.4
Completeness [%]	99.9
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	2.25 M (NH4)2SO4, 0.1M Bis-Tris