5SVZ

HIV-1 Tat NLS in complex with importin alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	79.000, 89.831, 99.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.662 - 2.000
R-factor	0.1752
Rwork	0.174
R-free	0.19240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fc8
RMSD bond length	0.003
RMSD bond angle	0.653
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.5.21)
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.662	29.660	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.095	0.028	1.057
Number of reflections	46564
<I/σ(I)>	13.4
Completeness [%]	96.5	88.6	98.2
Redundancy	4.6	4.3	4.6
CC(1/2)	0.998	0.999	0.650

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	1.25 M sodium citrate pH 7 and 10 mM DTT