5SVV
Structure and kinetics of the LOV domain of ZEITLUPE determine its circadian function in Arabidopsis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2014-03-06 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 85.427, 85.427, 198.775 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.367 - 2.101 |
| R-factor | 0.1649 |
| Rwork | 0.163 |
| R-free | 0.20020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d72 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.464 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.251 | |
| Number of reflections | 49510 | |
| <I/σ(I)> | 36.6 | 12.8 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 7.9 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Tris ph 8.5, 0.2 M Sodium Acetate trihydrate, 30% w/v PEG 4K |
