5SUS
X-ray crystallographic structure of a covalent trimer derived from A-beta 17_36. X-ray diffractometer data set. (ORN)CVF(MEA)CED(ORN)AIIGL(ORN)V.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 133 |
| Detector technology | CCD |
| Collection date | 2015-09-14 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 57.085, 57.085, 93.682 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.400 - 2.349 |
| R-factor | 0.2246 |
| Rwork | 0.219 |
| R-free | 0.27860 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.795 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.400 | 2.433 |
| High resolution limit [Å] | 2.349 | 2.349 |
| Rmerge | 0.009 | 0.041 |
| Number of reflections | 4143 | |
| <I/σ(I)> | 57.82 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296.15 | 0.1 M Tris buffer at pH 7.7, 0.2 M MgCl2, 3.45 M 1,6-hexanediol |
