5SUL
Inhibited state structure of yGsy2p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-06 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.919 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 122.447, 122.447, 279.361 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.040 - 3.300 |
| R-factor | 0.16119 |
| Rwork | 0.158 |
| R-free | 0.22298 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3naz |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.665 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.360 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.134 | 0.621 |
| Number of reflections | 35194 | |
| <I/σ(I)> | 7.59 | 2.13 |
| Completeness [%] | 99.4 | 99.5 |
| Redundancy | 4.8 | 5 |
| CC(1/2) | 0.703 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Peg 6000, Magnesium Chloride, Tris-HCl |
