5SB5

Tubulin-todalam-9-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-23
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00003
Spacegroup name	P 21 21 21
Unit cell lengths	104.810, 157.711, 180.536
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.550 - 2.310
R-factor	0.1898
Rwork	0.188
R-free	0.22040
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.19.1_4122)
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.550	49.550	2.370
High resolution limit [Å]	2.310	10.320	2.310
Rmerge	0.190	0.049	1.858
Rmeas	0.197	0.051	1.927
Total number of observations	1809337
Number of reflections	131912	1637	9601
<I/σ(I)>	11.45	42.41	1.45
Completeness [%]	99.9	98.7	99.5
Redundancy	13.716	12.579	14.019
CC(1/2)	0.998	0.998	0.583

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine