5S67

Tubulin-Z1896597864-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.900, 158.960, 179.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.750 - 2.100
R-factor	0.2116
Rwork	0.210
R-free	0.23780
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.750	62.750	2.150
High resolution limit [Å]	2.100	9.390	2.100
Rmerge	0.087	0.027	2.708
Rmeas	0.094	0.030	2.973
Total number of observations	1150032
Number of reflections	172579	2155	12791
<I/σ(I)>	11.66	51.68	0.63
Completeness [%]	98.4	99.3	99.7
Redundancy	6.664	6.039	5.95
CC(1/2)	0.999	0.999	0.245

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine