5S66

Tubulin-Z2856434929-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.690, 157.880, 178.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.000 - 2.100
R-factor	0.2154
Rwork	0.214
R-free	0.24350
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	68.000	68.000	2.150
High resolution limit [Å]	2.100	9.390	2.100
Rmerge	0.084	0.025	2.771
Rmeas	0.091	0.027	3.040
Total number of observations	1159094
Number of reflections	172450	2123	12586
<I/σ(I)>	12.19	57.69	0.61
Completeness [%]	99.8	99.5	99.5
Redundancy	6.721	6.227	5.956
CC(1/2)	0.999	0.999	0.255

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine