5S63

Tubulin-Z2241115980-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	105.220, 159.100, 179.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.780 - 2.600
R-factor	0.2155
Rwork	0.213
R-free	0.26800
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.780	62.780	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.136	0.029	2.809
Rmeas	0.147	0.032	3.064
Total number of observations	604231
Number of reflections	91696	1166	6743
<I/σ(I)>	10.15	45.37	0.78
Completeness [%]	98.3	98.8	98.7
Redundancy	6.59	6.3	6.31
CC(1/2)	0.998	0.999	0.377

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine