5S62

Tubulin-Z100642432-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	105.480, 159.440, 179.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.600 - 2.750
R-factor	0.2133
Rwork	0.210
R-free	0.26690
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.600	63.600	2.820
High resolution limit [Å]	2.750	12.300	2.750
Rmerge	0.116	0.023	3.151
Rmeas	0.125	0.025	3.410
Total number of observations	516508
Number of reflections	76457	997	5751
<I/σ(I)>	12	61.41	0.62
Completeness [%]	96.2	98.6	98.9
Redundancy	6.756	6.257	6.9
CC(1/2)	0.999	0.999	0.328

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine