5S5Z

Tubulin-Z2856434944-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	105.180, 158.030, 182.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	91.080 - 2.550
R-factor	0.2111
Rwork	0.209
R-free	0.25250
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	91.080	91.080	2.620
High resolution limit [Å]	2.550	11.400	2.550
Rmerge	0.122	0.031	2.389
Rmeas	0.133	0.034	2.590
Total number of observations	668786
Number of reflections	98956	1234	7237
<I/σ(I)>	9.64	44.6	0.75
Completeness [%]	99.5	99	99.8
Redundancy	6.758	6.262	6.711
CC(1/2)	0.999	0.999	0.326

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine