5S5Y

Tubulin-Z26781952-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.960, 158.750, 179.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.310 - 2.260
R-factor	0.203
Rwork	0.202
R-free	0.23130
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.310	63.310	2.320
High resolution limit [Å]	2.260	10.110	2.260
Rmerge	0.076	0.024	1.892
Rmeas	0.082	0.027	2.042
Total number of observations	955638
Number of reflections	140440	1738	10214
<I/σ(I)>	14.08	58.18	1.06
Completeness [%]	99.9	99	99.8
Redundancy	6.805	6.27	7.078
CC(1/2)	0.999	0.999	0.491

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine