5S5X

Tubulin-Z45705015-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.960, 159.130, 178.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.410 - 2.320
R-factor	0.2091
Rwork	0.207
R-free	0.24320
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.410	63.410	2.380
High resolution limit [Å]	2.320	10.380	2.320
Rmerge	0.112	0.027	2.412
Rmeas	0.122	0.029	2.605
Total number of observations	875360
Number of reflections	128879	1608	9494
<I/σ(I)>	10.35	51.6	0.77
Completeness [%]	99.4	99.3	99.7
Redundancy	6.792	6.285	7.052
CC(1/2)	0.999	0.999	0.349

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine