5S5V

Tubulin-Z32386228-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	105.230, 159.540, 179.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.870 - 2.700
R-factor	0.2191
Rwork	0.217
R-free	0.26700
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	72.870	72.870	2.770
High resolution limit [Å]	2.700	12.070	2.700
Rmerge	0.128	0.017	3.274
Rmeas	0.139	0.019	3.558
Total number of observations	560794
Number of reflections	82746	1036	6052
<I/σ(I)>	11.15	69.9	0.58
Completeness [%]	99.2	98.1	99.6
Redundancy	6.777	6.403	6.572
CC(1/2)	0.999	1.000	0.230

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine