5S5R

Tubulin-Z33452106-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	105.240, 159.480, 178.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.810 - 2.300
R-factor	0.2083
Rwork	0.206
R-free	0.24390
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	72.810	72.810	2.360
High resolution limit [Å]	2.300	10.290	2.300
Rmerge	0.092	0.029	2.512
Rmeas	0.100	0.032	2.711
Total number of observations	896575
Number of reflections	132891	1650	9791
<I/σ(I)>	11.04	46.18	0.81
Completeness [%]	99.4	98.9	99.8
Redundancy	6.747	5.968	7.063
CC(1/2)	0.999	0.999	0.257

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine