5S5P

Tubulin-Z53825177-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	105.710, 160.630, 179.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	80.320 - 2.790
R-factor	0.2262
Rwork	0.224
R-free	0.26470
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	80.320	80.320	2.860
High resolution limit [Å]	2.790	12.480	2.790
Rmerge	0.105	0.019	2.532
Rmeas	0.113	0.021	2.729
Total number of observations	514883
Number of reflections	75930	958	5567
<I/σ(I)>	11.93	62.16	0.79
Completeness [%]	99.2	98	99.2
Redundancy	6.781	6.223	7.098
CC(1/2)	0.999	0.999	0.368

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine