5S5M

Tubulin-Z45527714-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	105.660, 159.800, 179.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.920 - 2.700
R-factor	0.2177
Rwork	0.215
R-free	0.26930
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.920	55.920	2.770
High resolution limit [Å]	2.700	12.070	2.700
Rmerge	0.100	0.022	2.798
Rmeas	0.109	0.025	3.039
Total number of observations	565536
Number of reflections	83292	1042	6020
<I/σ(I)>	12.72	63.97	0.59
Completeness [%]	99.3	98.1	98.7
Redundancy	6.79	6.371	6.59
CC(1/2)	0.999	1.000	0.321

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine