5S5J

Tubulin-Z1148747945-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.770, 159.730, 179.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.710 - 2.250
R-factor	0.2064
Rwork	0.205
R-free	0.23810
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.710	62.710	2.310
High resolution limit [Å]	2.250	10.080	2.250
Rmerge	0.099	0.040	2.195
Rmeas	0.108	0.044	2.377
Total number of observations	953676
Number of reflections	140823	1746	10309
<I/σ(I)>	10.3	39.18	0.92
Completeness [%]	99.0	98.9	98.7
Redundancy	6.772	6.263	6.855
CC(1/2)	0.998	0.998	0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine