5S5H

Tubulin-Z2074076908-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.910, 158.890, 179.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	90.540 - 2.500
R-factor	0.219
Rwork	0.216
R-free	0.26980
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	90.540	90.540	2.560
High resolution limit [Å]	2.500	11.180	2.500
Rmerge	0.124	0.019	4.031
Rmeas	0.135	0.021	4.364
Total number of observations	693010
Number of reflections	103319	1299	7511
<I/σ(I)>	10.91	67	0.51
Completeness [%]	99.3	99	98.7
Redundancy	6.707	6.271	6.808
CC(1/2)	0.999	0.999	0.226

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine