5S5G

Tubulin-Z1129283193-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.645, 157.922, 179.187
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.260 - 2.690
R-factor	0.2188
Rwork	0.217
R-free	0.27120
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	72.260	72.260	3.000
High resolution limit [Å]	2.930	13.100	2.930
Rmerge	0.178	0.083	0.851
Rmeas	0.193	0.090	0.918
Total number of observations	443945
Number of reflections	65335	752	4671
<I/σ(I)>	7.72	17.47	2.21
Completeness [%]	99.5	88.6	97.7
Redundancy	6.795	6.352	6.991
CC(1/2)	0.989	0.992	0.766

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine