5S5E

Tubulin-Z1515654336-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	105.407, 159.503, 179.362
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.760 - 2.670
R-factor	0.1875
Rwork	0.186
R-free	0.22700
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.760	29.760	2.740
High resolution limit [Å]	2.670	11.940	2.670
Rmerge	0.096	0.049	0.796
Rmeas	0.104	0.053	0.862
Total number of observations	581871
Number of reflections	86229	1072	6285
<I/σ(I)>	12.73	33.14	2.5
Completeness [%]	99.9	98.5	100
Redundancy	6.748	6.366	6.714
CC(1/2)	0.997	0.996	0.789

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine