5S5D

Tubulin-Z32400357-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	105.070, 159.080, 179.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.420 - 1.900
R-factor	0.2012
Rwork	0.200
R-free	0.22700
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.420	63.420	1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.065	0.036	1.797
Rmeas	0.070	0.039	2.022
Total number of observations	1497208
Number of reflections	235320	2884	17248
<I/σ(I)>	12.64	43.59	0.74
Completeness [%]	99.9	99.4	99.5
Redundancy	6.362	6.128	4.845
CC(1/2)	0.999	0.997	0.309

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine