5S5A

Tubulin-Z1449748885-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.590, 158.160, 179.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.080 - 2.350
R-factor	0.2072
Rwork	0.205
R-free	0.23720
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.080	63.080	2.410
High resolution limit [Å]	2.350	10.510	2.350
Rmerge	0.086	0.016	2.276
Rmeas	0.093	0.017	2.458
Total number of observations	840243
Number of reflections	124084	1538	9069
<I/σ(I)>	14.44	84.39	0.85
Completeness [%]	99.8	99.3	99.8
Redundancy	6.772	6.277	7.027
CC(1/2)	0.999	1.000	0.398

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine